Coding

Part:BBa_K3765001:Design

Designed by: Dipayan Akhuli   Group: iGEM21_IISc-Bangalore   (2021-10-14)


SpyCatcher002


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 150
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 235


Design Notes

Codon optimized for E. coli.

Source

Modified protein fragment. Engineered from the second immunoglobulin-like collagen adhesin domain (CnaB2) of the fibronectin binding protein FbaB from Streptococcus pyogenes.

References

  1. Zakeri, B. et al. Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin. Proc. Natl Acad. Sci. USA 109, E690–E697 (2012).
  2. Sutherland, A. R., Alam, M. K. & Geyer, C. R. Post-translation assembly of protein parts into complex devices using SpyTag/SpyCatcher protein ligase. Chembiochem 20, 319–328 (2019).
  3. Keeble, A. H. et al. Evolving accelerated amidation by SpyTag/SpyCatcher to analyze membrane dynamics. Angew. Chem. Int. Ed. 56, 16521–16525 (2017).